Rat mTOR (Serine/threonine-protein kinase mTOR) ELISA Kit
Artikelnummer:
EKL-ER22176
Hersteller Artikelnummer:
ER22176
Alternativnummer:
EKL-ER22176-96T
Hersteller:
EnkiLife
Kategorie:
Kits/Assays
Spezies Reaktivität:
Rat
Alternative Synonym:
FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1, FK506 Binding Protein 12 Rapamycin Associated Protein, Mammalian Target Of Rapamycin, FKBP12-Rapamycin Complex-Associated Protein 1, Rapamycin and FKBP12 target
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat mTOR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat mTOR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat mTOR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat mTOR. You can calculate the concentration of Rat mTOR in the samples by comparing the OD of the samples to the standard curve.
Detektionsbereich:
0.15-10ng/mL
Sensitivitaet:
0.062ng/mL
Proben:
Serum,plasma and other biological fluids
Target-Kategorie:
mTOR
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
