Siglecs are sialic acid specific I-type lectins that are characterized by an extracellular domain (ECD) with an N-terminal Ig-like V-set domain followed by varying numbers of Ig-like C2-set domains. Mouse Siglec-E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 - 85 kDa member of the CD33-related subfamily of Siglecs. Rodent and primate Siglec gene families have significantly diverged, and Siglec-9 is the most likely human ortholog of mouse Siglec-E. Siglec-E is expressed as a heavily N-glycosylated disulfide-linked homodimer and shows binding preference for disialic acids in the alpha 2-8 linkage. Siglec-E is up-regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists. Siglec-E signaling negatively regulates the LPS-induced production of TNF- alpha and IL-6 by macrophages. Its up-regulation in macrophages parallels the development of endotoxin tolerance. Siglec-E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL-12 p40 production.
Greater than 95% as determined by reducing SDS-PAGE
Anwendungsbeschreibung:
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100µg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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