CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Klon-Bezeichnung:
[1H4]
Isotyp:
Mouse IgG2b
Puffer:
Phosphate buffered saline (PBS), pH 7.4
Lagerung:
2°C to 8°C
Target-Kategorie:
CD54
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunohistochemistry (frozen sections): Recommended dilution: 5-10 µg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 µg/ml, prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required. Flow cytometry: Recommended dilution: 1-4 µg/ml.Western blotting: Recommended dilution: 1-2 µg/ml, non-reducing conditions.
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