CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Klon-Bezeichnung:
[GK1.5]
Isotyp:
Rat IgG2b
Puffer:
Phosphate buffered saline (PBS), pH 7.4
Lagerung:
2°C to 8°C
Target-Kategorie:
CD4
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4. Immunocytochemistry: Recommended dilution: 1-4 µg/ml. Immunoprecipitation: Recommended dilution: 1-2 µg / 100-500 µg of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 µg/million cells. Immunohistochemistry: Recommended dilution: 5-10 µg/ml.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
