Cytoskeleton preparation of epidermal carcinoma cell line A431.
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Klon-Bezeichnung:
[C-04]
Isotyp:
Mouse IgG1
Puffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Lagerung:
2°C to 8°C
Target-Kategorie:
Cytokeratin 18
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western blotting: Recommended dilution: 1-2 µg/ml.Immunohistochemistry (paraffin sections): Recommended dilution: 10 µg/ml, positive control: colon, kidney. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of cytokeratin 18 in combination with the antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
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