Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Klon-Bezeichnung:
[B2M-01]
Isotyp:
Mouse IgG2a
Puffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Lagerung:
2°C to 8°C
Target-Kategorie:
beta2-Microglobulin
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western blotting: Recommended dilution: 2-4 µg/ml, positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line, both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 µg/ml, positive tissue: kidney glomeruli. Flow cytometry: Recommended dilution: 0.5-4 µg/ml, positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. ELISA: Working dilution should be determinated by investigator. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
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