Anti-c-Myc tag Purified, Clone: [9E10], Monoclonal

Produktübersicht

• Artikelname: Anti-c-Myc tag Purified, Clone: [9E10], Monoclonal
• Artikelnummer: EXB-11-433-C100
• Hersteller Artikelnummer: 11-433-C100
• Alternativnummer: EXB-11-433-C100
• Hersteller: EXBIO
• Kategorie: Antikörper
• Applikation: ELISA, FC, ICC, IHC-Fr, IHC-P, IP
• Spezies Reaktivität: All, Human
• Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.

Produktbeschreibung

The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml
• Klon-Bezeichnung: [9E10]
• Isotyp: Mouse IgG1
• Puffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Lagerung: 2°C to 8°C
• Target-Kategorie: c-Myc tag
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Anwendungsbeschreibung: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 µg/ml, positive tissue: perfused brain sections, liver, spleen. Immunoprecipitation: Recommended dilution: 1-5 µg/ml, this antibody is not suitable for immunoprecipitation of native c-Myc protein. Flow cytometry: Intracellular or extracellular staining, depending on particular expression. Recommended dilution: 1-4 µg/ml.Western blotting: Recommended dilution: 0,5-2 µg/ml, positive control: c-Myc tagged protein.