Bovine, Gallus, Human, Mouse, Plant, Porcine, Protozoa, Rat
Immunogen:
C-terminal peptide of gamma-tubulin counjugated to KLH.
The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.
Klonalität:
Monoclonal
Konzentration:
1 mg/ml
Klon-Bezeichnung:
[TU-30]
Isotyp:
Mouse IgG1
Puffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Lagerung:
2°C to 8°C
Target-Kategorie:
gamma-Tubulin
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunocytochemistry: Recommended dilution: 1-2 µg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly in methanol for 10 min at -20C or in acetone for 10 min at -20C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.Western blotting: Recommended dilution 1-2 µg/ml, reducing conditions.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
