Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3-hydroxyl terminus of oligonucleotide primers. It is expressed in restricted populations of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.
Flow cytometry: The reagent is designed for intracellular analysis of human blood cells after XPerm (fixation and permeabilization reagent, <a href=https://www.exbio.cz/clinical-products/reproductive-immunology/lysing-solutions/excellyse-xperm-1 target=_blank>ED7397) permeabilization of cell membrane and using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
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