SP1 antibody [JF0950]

Artikelnummer: GTX-GTX01012
Artikelname: SP1 antibody [JF0950]
Artikelnummer: GTX-GTX01012
Hersteller Artikelnummer: GTX01012
Alternativnummer: GTX01012-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein
Konjugation: Unconjugated
Alternative Synonym: specificity protein 1, transcription factor Sp1
Klonalität: Monoclonal
Konzentration: 1 mg/ml
Klon-Bezeichnung: JF0950
Isotyp: IgG
NCBI: 6667
UniProt: P08047
Puffer: 1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide
Reinheit: Protein A purified
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: Sp1 transcription factor
Anwendungsbeschreibung: WB: 1:1000-1:2000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ICC/IF analysis of PFA-fixed HeLa cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
IHC-P analysis of human kidney tissue section using GTX01012 SP1 antibody [JF0950].
ICC/IF analysis of PFA-fixed SW480 cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01012 SP1 antibody [JF0950].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
IHC-P analysis of human colon cancer tissue section using GTX01012 SP1 antibody [JF0950].
IHC-P analysis of human breast carcinoma tissue section using GTX01012 SP1 antibody [JF0950].