GNAO1 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX114439
Artikelname: GNAO1 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX114439
Hersteller Artikelnummer: GTX114439
Alternativnummer: GTX114439-25,GTX114439-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IP, ICC, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Human, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human GNAO1. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: G protein subunit alpha o1 , EIEE17 , G-ALPHA-o , GNAO , HLA-DQB1 , NEDIM
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 40
Isotyp: IgG
NCBI: 2775
UniProt: P09471
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: G protein subunit alpha o1
Application Verdünnung: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 μg of GNAO1 antibody (GTX114439).
Western blot analysis was performed using GNAO1 antibody (GTX114439).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GNAO1 antibody detects GNAO1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GNAO1 antibody (GTX114439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GNAO1 antibody detects GNAO1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
GNAO1 antibody (GTX114439) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
GNAO1 antibody (GTX114439) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min