Galectin 1 antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelname:	Galectin 1 antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	GTX-GTX116411
Hersteller Artikelnummer:	GTX116411
Alternativnummer:	GTX116411-25,GTX116411-100
Hersteller:	GeneTex International
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	WB, IP, ICC
Spezies Reaktivität:	Mouse, Human, Rat
Immunogen:	Full length human Galectin 1 Recombinant protein.
Konjugation:	Unconjugated
Alternative Synonym:	galectin 1 , GAL1 , GBP

Klonalität:	Polyclonal
Konzentration:	1 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht:	15
Isotyp:	IgG
NCBI:	3956
UniProt:	P09382
Puffer:	0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit:	Purified by antigen-affinity chromatography.
Lagerung:	2°C to 8°C, -20°C or -80°C
Target-Kategorie:	galectin 1

Application Verdünnung:	WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung:	WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

	Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Sample (30 μg of whole cell lysate) A: NCI-H929 15% SDS PAGE GTX116411 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Sample (30 μg of whole cell lysate) A: BCL-1 15% SDS PAGE GTX116411 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Immunofluorescence analysis of methanol-fixed A431, using Galectin 1(GTX116411) antibody at 1:200 dilution.
	Galectin 1 antibody detects Galectin 1 protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 15% SDS-PAGE Galectin 1 antibody (GTX116411) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments._x000D_IP Sample: HeLa whole cell lysate/extract_x000D_A : 30 μg whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells_x000D_B : Control with 2.5 μg of pre-immune rabbit IgG_x000D_C : Immunoprecipitation of Galectin 1 by 2.5 μg of Galectin 1 antibody (GTX116411) _x000D_15% SDS-PAGE _x000D_The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody (GTX116411) diluted at 1 : 1000. _x000D_EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
	Galectin 1 antibody detects Galectin 1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
	Wild-type (WT) and Galectin 1 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
	Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.