1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit:
Purified by antigen-affinity chromatography.
Lagerung:
2°C to 8°C, -20°C or -80°C
Target-Kategorie:
myosin XIX
Anwendungsbeschreibung:
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Immunohistochemical analysis of paraffin-embedded mouse muscle, using MYO19(GTX122503) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 7.5% SDS PAGE GTX122503 diluted at 1:3000
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using MYO19(GTX122503) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
MYO19 antibody [N1N2], N-term detects MYO19 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: MYO19 protein stained by MYO19 antibody [N1N2], N-term (GTX122503) diluted at 1:500. Blue: Hoechst 33342 staining.
MYO19 antibody [N1N2], N-term detects MYO19 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MYO19 antibody [N1N2], N-term (GTX122503) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten