CHMP4C antibody

Artikelnummer: GTX-GTX122876
Artikelname: CHMP4C antibody
Artikelnummer: GTX-GTX122876
Hersteller Artikelnummer: GTX122876
Alternativnummer: GTX122876-25,GTX122876-100
Hersteller: Genetex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Recombinant protein encompassing a sequence within the center region of human CHMP4C. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: charged multivesicular body protein 4c, chromatin modifying protein 4C, chromatin-modifying protein 4c, hSnf7-3, hVps32-3, Shax3, SNF7 homolog associated with Alix 3, Snf7 homologue associated with Alix 3, SNF7-3, vacuolar protein sorting-associated protein 32-3, vps32-3, VPS32C
Klonalität: Polyclonal
Konzentration: 1 mg/ml
Isotyp: IgG
NCBI: 92421
UniProt: Q96CF2
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: charged multivesicular body protein 4C
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Sample (50 ug of whole cell lysate)
A: mouse kidney
12% SDS PAGE
GTX122876 diluted at 1:1000
Sample (20 ug of whole cell lysate)
A: HeLa
B: HeLa cytoplasm
12% SDS PAGE
GTX122876 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using CHMP4C(GTX122876) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP4C antibody (GTX122876) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CHMP4C antibody detects CHMP4C protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CHMP4C stained by CHMP4C antibody (GTX122876) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.