Dengue virus NS4B protein antibody, IgG, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX-GTX124250
Artikelname: Dengue virus NS4B protein antibody, IgG, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX-GTX124250
Hersteller Artikelnummer: GTX124250
Alternativnummer: GTX124250-25,GTX124250-100
Hersteller: GeneTex International
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FACS, WB, ICC
Spezies Reaktivität: Virus
Immunogen: Full length Dengue virus 2 NS4B Recombinant protein
Konjugation: Unconjugated
Alternative Synonym: Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 27
Isotyp: IgG
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: Nonstructural protein NS4B
Application Verdünnung: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. FACS: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. FACS: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
PMID: 25412261"> The WB analysis of Dengue virus NS4B protein antibody was published by Tsai TT and colleagues in the journal PLoS Negl Trop Dis in 2014.PMID: 25412261
PMID: 25412261"> The WB analysis of Dengue virus NS4B protein antibody was published by Tsai TT and colleagues in the journal PLoS Negl Trop Dis in 2014.PMID: 25412261
PMID: 25412261"> The WB analysis of Dengue virus NS4B protein antibody was published by Tsai TT and colleagues in the journal PLoS Negl Trop Dis in 2014.PMID: 25412261
Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
10% SDS PAGE
GTX124250 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX124250) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution.