Dengue virus NS4B protein antibody, IgG, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Dengue virus NS4B protein antibody, IgG, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX-GTX124250
• Hersteller Artikelnummer: GTX124250
• Alternativnummer: GTX124250-25,GTX124250-100
• Hersteller: GeneTex International
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FACS, WB, ICC
• Spezies Reaktivität: Virus
• Immunogen: Full length Dengue virus 2 NS4B Recombinant protein
• Konjugation: Unconjugated
• Alternative Synonym: Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 27
• Isotyp: IgG
• Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Reinheit: Purified by antigen-affinity chromatography.
• Lagerung: 2°C to 8°C, -20°C or -80°C
• Target-Kategorie: Nonstructural protein NS4B

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. FACS: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. FACS: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

• PMID: 25412261">: The WB analysis of Dengue virus NS4B protein antibody was published by Tsai TT and colleagues in the journal PLoS Negl Trop Dis in 2014.PMID: 25412261
• PMID: 25412261">: The WB analysis of Dengue virus NS4B protein antibody was published by Tsai TT and colleagues in the journal PLoS Negl Trop Dis in 2014.PMID: 25412261
• PMID: 25412261">: The WB analysis of Dengue virus NS4B protein antibody was published by Tsai TT and colleagues in the journal PLoS Negl Trop Dis in 2014.PMID: 25412261

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX124250) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
10% SDS PAGE
GTX124250 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX124250) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX124250) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS4B (Dengue virus 2)(GTX124250) antibody at 1:2000 dilution.