Neurofibromin antibody [McNFn27b]

Artikelnummer: GTX-GTX15776
Artikelname: Neurofibromin antibody [McNFn27b]
Artikelnummer: GTX-GTX15776
Hersteller Artikelnummer: GTX15776
Alternativnummer: GTX15776-100
Hersteller: Genetex
Wirt: Mouse
Kategorie: Antikörper
Applikation: ICC, IF, IHC, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
Konjugation: Unconjugated
Alternative Synonym: neurofibromatosis 1, neurofibromatosis-related protein NF-1, neurofibromin, NFNS, truncated neurofibromin 1, VRNF, WSS
Klonalität: Monoclonal
Konzentration: 1 mg/ml
Klon-Bezeichnung: McNFn27b
Isotyp: IgG1
NCBI: 4763
UniProt: P21359
Puffer: PBS, 1mg/ml BSA, 0.05% sodium azide
Reinheit: Protein G purified
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: neurofibromin 1
Anwendungsbeschreibung: WB: 1-3 µg/ml. ICC/IF: 1-2 µg/ml. IHC-P: 5-10 µg/mL. IHC: Assay dependent. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
ICC/IF analysis of NIH-3T3 cells using GTX15776 Neurofibromin antibody [McNFn27b]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
ICC/IF analysis of HeLa cells using GTX15776 Neurofibromin antibody [McNFn27b]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC
ICC/IF analysis of SH-SY5Y cells using GTX15776 Neurofibromin antibody [McNFn27b]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature
WB analysis of whole cell extracts (30 μg lysate) of MCF-7 (Lane 1) and HepG2 (Lane 2) using GTX15776 Neurofibromin antibody [McNFn27b].
Dilution : 1-3 μg/ml
IHC-P analysis of human stomach tissue using GTX15776 Neurofibromin antibody [McNFn27b].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
IHC-P analysis of human breast carcinoma tissue using GTX15776 Neurofibromin antibody [McNFn27b].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
ICC/IF analysis of U251 cells using GTX15776 Neurofibromin antibody [McNFn27b]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC