ELISA: Use at a concentration of 0.1 - 1.0 µg/ml. IHC: Use at a dilution of 1/100 - 1/400. WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 42 kDa. The formalin-fixed tissues were boiled in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.
IHC analysis of human leiomyoma using alpha Smooth Muscle Actin antibody at a dilution of 1:100. Antigen retrieval was performed by boiling in 10 mM citrate buffer, pH 6.0 for 10 min.
Western blot of 3T3 (A) and mouse heart whole tissue lysate (B) labeled with anti-Actin antibody alpha smooth muscle (GTX25694).
WB analysis of 3T3 lysate using alpha Smooth Muscle Actin antibody at a concentration of 1.0 μg/ml.
Immunocytochemistry of human dermal collagen in the skin (Formalin fixed/Paraffin-embedded) stained with GTX25703.
WB analysis of 3T3 lysate using alpha Smooth Muscle Actin antibody at a concentration of 1 ug/ml in the abscence (A) and prescence (B) of blocking peptide.
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