SignalPlus Antibody Enhancer

Artikelnummer: GTX-GTX49999
Artikelname: SignalPlus Antibody Enhancer
Artikelnummer: GTX-GTX49999
Hersteller Artikelnummer: GTX49999
Hersteller: Genetex
Kategorie: Biochemikalien
Applikation: DOT, ELISA, WB
Formulierung: Liquid
Lagerung: 2°C to 8°C
Anwendungsbeschreibung: Dilute primary antibody in Solution 1 and secondary antibody in Solution 2. We recommend using a 10-fold lower working concentration of primary antibody than is usually required for western blot. Optimal working dilutions should be determined experimentally by the end user.
 Detection enhancement of Rac1 with treatment of Signal + in Western blot. Treatment with GTX49999 resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal. Primary Ab: Anti-Rac1 (1: 1,000) Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500) Sample: Rat cortical primary neuron Detection: ECL
 Antibody diluted in SignalPlus Antibody Enhancer solution has superior signal to antibody diluted in standard buffers, even at a 10-fold lower concentration of antibody.
 Detection enhancement of pJNK with treatment of Signal + in Western blot. Treatment with GTX49999 resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T. Primary Ab: Anti-pJNK (1: 1,000) Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000) Sample: Mouse Embryonic Fibroblast (MEF) Detection: SuperSignal West Pico
 Antibody diluted in SignalPlus Antibody Enhancer solution can detect more than 100-fold lower levels of antigen than antibody diluted in standard buffers