SignalPlus Antibody Enhancer

Artikelnummer: GTX-GTX49999
Artikelname: SignalPlus Antibody Enhancer
Artikelnummer: GTX-GTX49999
Hersteller Artikelnummer: GTX49999
Alternativnummer: GTX49999-50
Hersteller: Genetex
Kategorie: Biochemikalien
Applikation: DOT, ELISA, WB
Lagerung: 2°C to 8°C
Anwendungsbeschreibung: Dilute primary antibody in Solution 1 and secondary antibody in Solution 2. We recommend using a 10-fold lower working concentration of primary antibody than is usually required for western blot. Optimal working dilutions should be determined experiment
Detection enhancement of Rac1 with treatment of Signal + in Western blot. Treatment with GTX49999 resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal. Primary Ab: Anti-Rac1 (1: 1,000) Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500) Sample: Rat cortical primary neuron Detection: ECL
Antibody diluted in SignalPlus Antibody Enhancer solution has superior signal to antibody diluted in standard buffers, even at a 10-fold lower concentration of antibody.
Detection enhancement of pJNK with treatment of Signal + in Western blot. Treatment with GTX49999 resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T. Primary Ab: Anti-pJNK (1: 1,000) Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000) Sample: Mouse Embryonic Fibroblast (MEF) Detection: SuperSignal West Pico
Antibody diluted in SignalPlus Antibody Enhancer solution can detect more than 100-fold lower levels of antigen than antibody diluted in standard buffers