Lamin A + C antibody [mab636], IgG2b, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: Lamin A + C antibody [mab636], IgG2b, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX-GTX80813
• Hersteller Artikelnummer: GTX80813
• Alternativnummer: GTX80813-200
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IHC-Fr, WB, IF, ICC
• Spezies Reaktivität: Bovine, Mouse, Human, Canine, Porcine
• Immunogen: Porcine lamin preparation.
• Konjugation: Unconjugated
• Alternative Synonym: lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: mab636
• Molekulargewicht: 74
• Isotyp: IgG2b
• NCBI: 4000
• UniProt: P02545
• Puffer: Tissue culture supernatant, 0.05% sodium azide
• Reinheit: Unpurified
• Lagerung: 2°C to 8°C, -20°C or -80°C
• Target-Kategorie: lamin A/C

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:100 - 1:2000. ICC/IF: 1:100. IHC-Fr: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
• Anwendungsbeschreibung: WB: 1:100 - 1:2000. ICC/IF: 1:100. IHC-Fr: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

ICC/IF analysis of A549 cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of U2-OS cells (A) or HeLa cells (B) using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

ICC/IF analysis of HeLa cells using GTX80813 Lamin A + C antibody [mab636].

ICC/IF analysis of U251 cells using GTX80813 Lamin A + C antibody [mab636]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

WB analysis of 10μg nuclear extract from fractionated mouse tissue lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:500

WB analysis of various amounts of human fibroblast whole cell lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:2000

ICC/IF analysis of MDCK cells using GTX80813 Lamin A + C antibody [mab636].
Red : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

ICC/IF analysis of HMVEC Cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of Human fibroblast cells using GTX80813 Lamin A + C antibody [mab636].
Fixation : methanol
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature