STAT1 antibody [4H9], IgG1, Unconjugated, Mouse, Monoclonal

Artikelnummer: GTX-GTX83552
Artikelname: STAT1 antibody [4H9], IgG1, Unconjugated, Mouse, Monoclonal
Artikelnummer: GTX-GTX83552
Hersteller Artikelnummer: GTX83552
Alternativnummer: GTX83552-100
Hersteller: GeneTex
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC-P, WB, IF, ICC
Spezies Reaktivität: Human, Canine, Monkey
Immunogen: Full length human recombinant protein of human STAT1 (NP_009330) produced in HEK293T cell.
Konjugation: Unconjugated
Alternative Synonym: signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF-3 , STAT91
Klonalität: Monoclonal
Konzentration: 0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: 4H9
Molekulargewicht: 87
Isotyp: IgG1
NCBI: 6772
UniProt: P42224
Puffer: PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Reinheit: Purified by affinity chromatography
Lagerung: 2°C to 8°C, -20°C or -80°C
Target-Kategorie: signal transducer and activator of transcription 1
Application Verdünnung: WB: 1:1000-1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Anwendungsbeschreibung: WB: 1:1000-1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83552 STAT1 antibody [4H9].
Loading : 10 ug per lane
Dilution : 1:200
IHC-P analysis of human breast tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human thyroid carcinoma tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
WB analysis of various cell lines using GTX83552 STAT1 antibody [4H9].
Loading : 35 ug per lane
ICC/IF analysis of HT29 cells using GTX83552 STAT1 antibody [4H9].
IHC-P analysis of human endometrium tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human kidney tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
ICC/IF analysis of COS7 cells transiently transfected with STAT1 plasmid using GTX83552 STAT1 antibody [4H9].
WB analysis of HEK293T cells transfected with STAT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83552 STAT1 antibody [4H9].
Loading : 5 ug per lane