Human Galectin 9 protein, His tag, Unconjugated
Artikelnummer:
GTX00111-PRO
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| Artikelname: | Human Galectin 9 protein, His tag, Unconjugated |
| Artikelnummer: | GTX00111-PRO |
| Hersteller Artikelnummer: | GTX00111-pro |
| Alternativnummer: | GTX00111-PRO-10 |
| Hersteller: | GeneTex |
| Kategorie: | Proteine/Peptide |
| Applikation: | FA |
| Spezies Reaktivität: | Human |
| Immunogen: | Met1~Thr323 |
| Konjugation: | Unconjugated |
| Alternative Synonym: | galectin 9 , HUAT , LGALS9A |
| Anwendungsbeschreibung: | GAL9 (Galectin-9) belongs to the galectin family, which is defined by their binding specificity for beta-galactoside sugars, such as N-acetyllactosamine (Galbeta1-3GlcNAc or Galbeta1-4GlcNAc). It is reported that GAL9 induces T-helper type 1 lymphocyte (Th1) death by binding to HAVCR2 (Hepatitis A virus cellular receptor 2), besides, the interaction between GAL9 and PDI (Protein disulfide-isomerase) leads to disulfide reductase activity increasing at the plasma membrane, therefore alters the plasma membrane redox state and enhances cell migration. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GAL9 with recombinant human HAVCR2 and recombinant human PDI separately. Briefly, GAL9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to HAVCR2-coated and PDI-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-GAL9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of GAL9 with HAVCR2 and PDI were shown in images, and this effect was in a dose dependent manner. |
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