Human IL10 Receptor beta protein, His tag, Unconjugated

Produktübersicht

• Artikelname: Human IL10 Receptor beta protein, His tag, Unconjugated
• Artikelnummer: GTX00131-PRO
• Hersteller Artikelnummer: GTX00131-pro
• Alternativnummer: GTX00131-PRO-10
• Hersteller: GeneTex
• Kategorie: Proteine/Peptide
• Applikation: FA
• Spezies Reaktivität: Human
• Konjugation: Unconjugated
• Alternative Synonym: interleukin 10 receptor subunit beta , CDW210B , CRF2-4 , CRFB4 , D21S58 , D21S66 , IL-10R2

Produkteigenschaften

• NCBI: 3588
• UniProt: Q08334
• Puffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose, ProClin 300.
• Expression System: E. coli
• Formulierung: Lyophilized powder
• Sequenz: N-terminal His-Tag, Leu87~Ala243 (NP_000619.3)

Anwendungsbeschreibung

• Anwendungsbeschreibung: IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IL10-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was in a dose dependent manner.