This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor su
Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl (pH8.0) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
E. coli
Formulierung:
Lyophilized powder
Sequenz:
N-terminal His-Tag, Glu21~Ala241 (NP_002599.1)
Anwendungsbeschreibung:
Platelet-derived growth factor subunit B (PDGFB) is a member of the platelet-derived growth factor family in humans. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines
GTX00204-pro Functional Assay Image
SDS-PAGE analysis of GTX00204-pro Human PDGF beta protein (active).
WB analysis of GTX00204-pro Human PDGF beta protein (active).
Cell proliferation effect of GTX00204-pro Human PDGF beta protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8). (A) Unstimulated MCF-7 cells cultured in DMEM for 96hrs. (B) MCF-7 cells cultured in DMEM, stimulated with 1 ng/ml PDGFB for 96hrs.
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