Mouse IL6 Receptor protein, His tag, Unconjugated

Produktübersicht

• Artikelname: Mouse IL6 Receptor protein, His tag, Unconjugated
• Artikelnummer: GTX00297-PRO
• Hersteller Artikelnummer: GTX00297-pro
• Alternativnummer: GTX00297-PRO-10
• Hersteller: GeneTex
• Kategorie: Proteine/Peptide
• Applikation: FA
• Spezies Reaktivität: Mouse
• Konjugation: Unconjugated
• Alternative Synonym: interleukin 6 receptor, alpha , CD126 , IL-6R , IL-6R-alpha , IL-6RA , Il6r

Produkteigenschaften

• NCBI: 16194
• UniProt: P22272
• Puffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose, ProClin 300.
• Expression System: E. coli
• Formulierung: Lyophilized powder
• Sequenz: N-terminal His-Tag, Ala52~Ala204 (NP_034689.2)

Anwendungsbeschreibung

• Anwendungsbeschreibung: IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IL6-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 µl stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was in a dose dependent manner.