NUP98 antibody [2H10], IgG2c, unconjugated, rat, Monoclonal

Artikelnummer: GTX00697
Artikelname: NUP98 antibody [2H10], IgG2c, unconjugated, rat, Monoclonal
Artikelnummer: GTX00697
Hersteller Artikelnummer: GTX00697
Alternativnummer: GTX00697-100
Hersteller: GeneTex
Wirt: rat
Kategorie: Antikörper
Applikation: wb, ICC, elisa, dot
Spezies Reaktivität: Yeast, rat, human, mouse, Fungi
Immunogen: Recombinant GST-fused human Nup98 (amino acids 1-466)
Konjugation: unconjugated
Alternative Synonym: nucleoporin 98 | ADIR2 | NUP196 | NUP96
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and
Klonalität: Monoclonal
Konzentration: 1mg/ml
Klon-Bezeichnung: 2H10
Molekulargewicht: 198
Isotyp: IgG2c
NCBI: 4928
Pubmed: 23607342, 16225424,
UniProt: P52948
Puffer: Filter-sterilized PBS, 50% Glycerol
Quelle: Human
Reinheit: Purified IgG
ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies.
Green : Primary antibody
Violet : DAPI
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.
WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml
WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml