LC3B antibody [GT1187], unconjugated, rabbit, Monoclonal

Artikelnummer: GTX00949
Artikelname: LC3B antibody [GT1187], unconjugated, rabbit, Monoclonal
Artikelnummer: GTX00949
Hersteller Artikelnummer: GTX00949
Alternativnummer: GTX00949-100
Hersteller: GeneTex
Wirt: rabbit
Kategorie: Antikörper
Applikation: wb, ICC, ihc-p
Spezies Reaktivität: human, rat, mouse
Immunogen: A synthetic peptide of human LC3B.
Konjugation: unconjugated
Alternative Synonym: microtubule associated protein 1 light chain 3 beta | ATG8F | LC3B | MAP1A/1BLC3 | MAP1LC3B | MAP1LC3Ba | APG8b
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a proces
Klonalität: Monoclonal
Konzentration: Batch dependent
Klon-Bezeichnung: GT1187
Molekulargewicht: 15
Isotyp: IgG
NCBI: 81631
UniProt: Q9GZQ8
Puffer: PBS (pH7.3), 50% Glycerol
Quelle: Human
Anwendungsbeschreibung: WB (1:500 - 1:2000), ICC/IF (1:50 - 1:200), IHC-P (1:50 - 1:200)
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT1187] (GTX00949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IHC-P analysis of Rat brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
ICC/IF analysis of PFA-fixed C6 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated C6 cells
Left : C6 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated HeLa cells
Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
IHC-P analysis of Mouse spinal cord tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100
WB analysis of C6 and NIH-3T3 cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg
WB analysis of wild-type (WT) and LC3B knockout (KO) 293T cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Dilution : 1:1000
Loading : 25μg
ICC/IF analysis of PFA-fixed NIH-3T3 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187].
Right : untreated NIH-3T3 cells
Left : NIH-3T3 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100
IHC-P analysis of Human brain tissue section using GTX00949 LC3B antibody [GT1187].
Dilution : 1:100