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Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT1187] (GTX00949) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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IHC-P analysis of Rat brain tissue section using GTX00949 LC3B antibody [GT1187]. Dilution : 1:100 |
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ICC/IF analysis of PFA-fixed C6 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187]. Right : untreated C6 cells Left : C6 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment Orange : Primary antibody Blue : DAPI Dilution : 1:100 |
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ICC/IF analysis of PFA-fixed HeLa cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187]. Right : untreated HeLa cells Left : HeLa cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment Orange : Primary antibody Blue : DAPI Dilution : 1:100 |
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IHC-P analysis of Mouse spinal cord tissue section using GTX00949 LC3B antibody [GT1187]. Dilution : 1:100 |
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GTX00949 WB Image |
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WB analysis of wild-type (WT) and LC3B knockout (KO) 293T cell extracts with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187]. Dilution : 1:1000 Loading : 25μg |
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ICC/IF analysis of PFA-fixed NIH-3T3 cells with/without Chloroquine (50 μM, 37ºC, 20 hrs) treatment using GTX00949 LC3B antibody [GT1187]. Right : untreated NIH-3T3 cells Left : NIH-3T3 cells with 50 μM Chloroquine (20 hrs, 37ºC) treatment Orange : Primary antibody Blue : DAPI Dilution : 1:100 |
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IHC-P analysis of Human brain tissue section using GTX00949 LC3B antibody [GT1187]. Dilution : 1:100 |