Smoothened homolog antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX02530
Artikelname: Smoothened homolog antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX02530
Hersteller Artikelnummer: GTX02530
Alternativnummer: GTX02530-100
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human SMO, corresponding to a region within N-terminal amino acids.
Konjugation: Unconjugated
Alternative Synonym: smoothened, frizzled class receptor , CRJS , FZD11 , Gx , SMOH
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein com
Klonalität: Polyclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 86
Isotyp: IgG
NCBI: 6608
UniProt: Q99835
Puffer: PBS pH7.4, 150mM NaCl, 50% Glycerol, 0.02% Sodium azide.
Quelle: Human
Reinheit: Purified by antigen-affinity chromatography
Formulierung: Liquid
Anwendungsbeschreibung: WB: 1:500-1:1000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
ICC/IF analysis of PFA-fixed HUVEC cells using GTX02530 Smoothened homolog antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
GTX02530 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX02530 Smoothened homolog antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
IHC-P analysis of human colorectal cancer tissue using GTX02530 Smoothened homolog antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
WB analysis of extracts from HUVEC cells using GTX02530 SMO antibody. The lane on the left was treated with the antigen-specific peptide.
IHC-P analysis of rat skin tissue using GTX02530 Smoothened homolog antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100