LDHA antibody, IgG, Clone: Polyclonal, Unconjugated, Human

Artikelnummer: GTX101416
Artikelname: LDHA antibody, IgG, Clone: Polyclonal, Unconjugated, Human
Artikelnummer: GTX101416
Hersteller Artikelnummer: GTX101416
Alternativnummer: GTX101416-100,GTX101416-25
Hersteller: GeneTex
Wirt: Human
Kategorie: Antikörper
Applikation: WB, IHC-P, ICC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: lactate dehydrogenase A , GSD11 , HEL-S-133P , LDHM , PIG19
Klonalität: Polyclonal
Konzentration: 0.48 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 37
Isotyp: IgG
NCBI: 3939
UniProt: P00338
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Immunofluorescence analysis of methanol-fixed A431, using LDHA(GTX101416) antibody at 1:500 dilution.
LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDHA protein stained by LDHA antibody (GTX101416) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using LDHA(GTX101416) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
LDHA antibody detects LDHA protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (GTX101416) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LDHA antibody detects LDHA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody (GTX101416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LDHA antibody detects LDHA protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
LDHA antibody (GTX101416) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LDHA antibody (GTX101416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LDHA antibody detects LDHA protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
LDHA stained by LDHA antibody (GTX101416) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min