p63 antibody [N2C1], Internal, IgG, Clone: Polyclonal, Unconjugated, Human

Artikelnummer: GTX102425
Artikelname: p63 antibody [N2C1], Internal, IgG, Clone: Polyclonal, Unconjugated, Human
Artikelnummer: GTX102425
Hersteller Artikelnummer: GTX102425
Alternativnummer: GTX102425-100,GTX102425-25
Hersteller: GeneTex
Wirt: Human
Kategorie: Antikörper
Applikation: IHC-Fr, PLA, ICC, WB, IHC-P, IP
Spezies Reaktivität: Human, Mouse, Rat, Canine
Immunogen: Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L
Klonalität: Polyclonal
Konzentration: 0.34 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 77
Isotyp: IgG
NCBI: 8626
UniProt: Q9H3D4
Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.
Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4ºC and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using p63(GTX102425) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation of p63 protein from A431 whole cell extracts using 5 μg of p63 antibody [N2C1], Internal (GTX102425).
Western blot analysis was performed using p63 antibody [N2C1], Internal (GTX102425).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
7.5% SDS-PAGE
p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
p63 antibody [N2C1], Internal (GTX102425) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tongue.
p63 stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min