Calretinin antibody, IgG, Clone: Polyclonal, Unconjugated, Human

Artikelnummer: GTX103261
Artikelname: Calretinin antibody, IgG, Clone: Polyclonal, Unconjugated, Human
Artikelnummer: GTX103261
Hersteller Artikelnummer: GTX103261
Alternativnummer: GTX103261-100,GTX103261-25
Hersteller: GeneTex
Wirt: Human
Kategorie: Antikörper
Applikation: WB, IHC-Fr, IHC-P, ICC
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: calbindin 2 , CAB29 , CAL2 , CR
Klonalität: Polyclonal
Konzentration: 0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 32
Isotyp: IgG
NCBI: 794
UniProt: P22676
Puffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Calretinin antibody detects Calretinin protein by immunohistochemical analysis.
Samples: Frozen Section adult mouse hippocampus.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Calretinin antibody detects CALB2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human CALB2-transfected 293T cells
12% SDS-PAGE
Calretinin antibody (GTX103261) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Calretinin antibody (GTX103261) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin(GTX103261) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
Calretinin antibody
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody (GTX103261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:200.
Blue: Hoechst 33342 staining.
Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).