CD2AP antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CD2AP antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX106235
• Hersteller Artikelnummer: GTX106235
• Alternativnummer: GTX106235-100,GTX106235-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CD2AP. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: CD2 associated protein , CMS

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.17 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 71
• NCBI: 23607
• UniProt: Q9Y5K6
• Puffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX106235 WB Image

CD2AP antibody detects CD2AP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
CD2AP antibody (GTX106235) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX106235 diluted at 1:1000

CD2AP antibody detects CD2AP protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD2AP antibody (GTX106235) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using CD2AP(GTX106235) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using CD2-associated protein(GTX106235) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD2AP antibody (GTX106235) diluted at 1:1000 and competitor's antibody (sc-9137) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD2AP antibody (GTX106235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

CD2AP antibody detects CD2AP protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD2AP stained by CD2AP antibody (GTX106235) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).