GFAP antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX108711
Artikelname: GFAP antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX108711
Hersteller Artikelnummer: GTX108711
Alternativnummer: GTX108711-100,GTX108711-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human GFAP. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: glial fibrillary acidic protein , ALXDRD
Klonalität: Polyclonal
Konzentration: 1.39 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 50
NCBI: 2670
UniProt: P14136
Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Anwendungsbeschreibung: WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX108711 WB Image
GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GFAP antibody detects GFAP protein at retinal ganglion cell layer by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
GFAP antibody detects GFAP protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: GFAP protein stained by GFAP antibody (GTX108711) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.
GFAP antibody detects GFAP protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: GFAP antibody (GTX108711) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GFAP antibody detects GFAP protein at glia cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GFAP antibody detects GFAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
GFAP stained by GFAP antibody (GTX108711) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GFAP antibody detects GFAP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: GFAP stained by GFAP antibody (GTX108711) diluted at 1:300.
Blue: Hoechst 33342 staining.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GFAP antibody (GTX108711) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.