Calnexin antibody [C3], C-term, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Calnexin antibody [C3], C-term, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX109669
• Hersteller Artikelnummer: GTX109669
• Alternativnummer: GTX109669-100,GTX109669-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat, Sheep
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: calnexin , CNX , IP90 , P90

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.46 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 68
• Sensitivitaet: IP/MS validation was supported by references (PMID:30377396)
• NCBI: 821
• UniProt: P27824
• Puffer: 1XPBS pH7, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Calnexin(GTX109669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Calnexin antibody immunoprecipitates Calnexin protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 30 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Calnexin protein by 2 μg of Calnexin antibody (GTX109669) 7.5% SDS-PAGE The immunoprecipitated Calnexin protein was detected by Calnexin antibody (GTX109669) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [C3], C-term detects Calnexin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:10000

Calnexin antibody [C3], C-term detects Calnexin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.