Coilin antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Coilin antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX112570
• Hersteller Artikelnummer: GTX112570
• Alternativnummer: GTX112570-100,GTX112570-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, IP, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Coilin. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: coilin , CLN80 , p80-coilin

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.66 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 63
• NCBI: 8161
• UniProt: P38432
• Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX112570 WB Image

Coilin antibody detects Coilin protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T nuclear extract
7.5 % SDS-PAGE
Coilin antibody (GTX112570) dilution: 1:1000

Coilin antibody detects Coilin protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Coilin antibody (GTX112570) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody (GTX112570).
Western blot analysis was performed using Coilin antibody (GTX112570).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Coilin(GTX112570) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.