beta 2 Microglobulin antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit

Artikelnummer: GTX112815
Artikelname: beta 2 Microglobulin antibody, IgG, Clone: Polyclonal, Unconjugated, Rabbit
Artikelnummer: GTX112815
Hersteller Artikelnummer: GTX112815
Alternativnummer: GTX112815-100,GTX112815-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, WB, IHC-P, IP
Spezies Reaktivität: Rat, Marmoset, Mouse, Human
Immunogen: Full length human beta 2 Microglobulin Recombinant protein.
Konjugation: Unconjugated
Alternative Synonym: beta-2-microglobulin , IMD43
Klonalität: Polyclonal
Konzentration: 0.83 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Polyclonal
Molekulargewicht: 14
Isotyp: IgG
NCBI: 567
UniProt: P61769
Puffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Reinheit: Purified by antigen-affinity chromatography.
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of B2M protein by 4 μg beta 2 Microglobulin antibody (GTX112815)
15 % SDS-PAGE
The immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Sample (30 ug of whole cell lysate)
A: A431
15% SDS PAGE
GTX112815 diluted at 1:5000
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta 2 Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta 2 Microglobulin stained by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Beta 2 Microglobulin antibody detects secreted B2M protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
B2M stained by Beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min