Influenza A virus Nucleoprotein antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Influenza A virus Nucleoprotein antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX125989
• Hersteller Artikelnummer: GTX125989
• Alternativnummer: GTX125989-100,GTX125989-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IHC-P, IP, WB
• Spezies Reaktivität: Virus
• Immunogen: Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: Influenza A Virus Nucleoprotein , Influenza A Virus NP

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.59 mg/ml (Please refer to the vial label for the specific concentration.)
• Puffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX125989 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by -% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) DF1 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Influenza A Virus NP antibody detects NP (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NP (H1N1) protein stained by Influenza A Virus NP antibody (GTX125989) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC,?30 min.