Dengue virus NS4A protein antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Dengue virus NS4A protein antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX132069
• Hersteller Artikelnummer: GTX132069
• Alternativnummer: GTX132069-100,GTX132069-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, WB
• Spezies Reaktivität: Virus
• Immunogen: Full length Dengue virus NS4A recombinant protein. (Dengue virus 2 (strain 16681 PDK 53))
• Konjugation: Unconjugated
• Alternative Synonym: Dengue Virus Non-structural protein 4A , Dengue Virus NS4A , DENV Non-structural protein 4A , DENV NS4A

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.12 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 16
• Puffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX132069 ICC/IF Image

Dengue virus NS4A protein antibody detects Dengue virus NS4A protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus NS4A protein stained by Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS4A protein antibody (GTX132069) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.