EasyBlot Sampler Kit

Produktübersicht

• Artikelname: EasyBlot Sampler Kit
• Artikelnummer: GTX300111
• Hersteller Artikelnummer: GTX300111
• Alternativnummer: GTX300111-1
• Hersteller: GeneTex
• Kategorie: Antikörper

Produktbeschreibung

EasyBlot anti-Rabbit/Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of rabbit/Mouse IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments.Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to mouse or rabbit IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian. This kit also includes an EasyBlocker (GTX425858) that can minimize the background caused by Protein A/G contamination. GeneTexs EasyBlot kit is simple to use and detects only the target band in your IP experiments, resulting in cleaner western blots.

Produkteigenschaften

• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: Suggested starting dilutions for WB is 1:1000. Use 2-5 µg of the IP antibody, do not exceed 5 µg. Completely reduce all IP samples by 100mM DTT (fresh prepared). For further detail, please refer to the protocol.Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.