K-Ras antibody [AT2F8], IgG2b, Unconjugated, Mouse, Monoclonal
Artikelnummer:
GTX31957
- Bilder (3)
| Artikelname: | K-Ras antibody [AT2F8], IgG2b, Unconjugated, Mouse, Monoclonal |
| Artikelnummer: | GTX31957 |
| Hersteller Artikelnummer: | GTX31957 |
| Alternativnummer: | GTX31957-100 |
| Hersteller: | GeneTex |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FACS, ICC, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | Recombinant human KRAS (1-186aa) purified from E. coli |
| Konjugation: | Unconjugated |
| Alternative Synonym: | KRAS proto-oncogene, GTPase , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2 |
| This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that re |
| Klonalität: | Monoclonal |
| Konzentration: | 1 mg/ml (Please refer to the vial label for the specific concentration.) |
| Klon-Bezeichnung: | [AT2F8] |
| Molekulargewicht: | 22 |
| Isotyp: | IgG2b |
| Sensitivitaet: | This antibody may cross react with human N-Ras and H-Ras. |
| NCBI: | 3845 |
| UniProt: | P01116 |
| Puffer: | PBS pH7.4, 10% Glycerol, 0.02% Sodium azide. |
| Quelle: | Human |
| Reinheit: | By protein-A affinity chromatography |
| Formulierung: | Liquid |
| Anwendungsbeschreibung: | WB: 1:500. ICC/IF: 1:100 |
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GTX31957 FACS Image |
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ICC/IF analysis of KRAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). |
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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : HepG2 cell lysateLane 3. : Ramos cell lysateLane 4. : A549 cell lysateLane 5. : Balb/3T3 cell lysate |
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