Trident RIPA Lysis Buffer

Artikelnummer: GTX400005
Artikelname: Trident RIPA Lysis Buffer
Artikelnummer: GTX400005
Hersteller Artikelnummer: GTX400005
Alternativnummer: GTX400005-100
Hersteller: GeneTex
Kategorie: Sonstiges
Applikation: WB
Radio-Immunoprecipitation assay lysis buffer
The ingredients of RIPA lysis buffer are as follows:
25 mM Tris pH 7.6
150 mM NaCl
1% Triton X-100
1% sodium deoxycholate
1% SDS
Formulierung: Liquid
Anwendungsbeschreibung: This product contains 1% SDS, which is higher than what is in some other commercial RIPA lysis buffers, and should facilitate a more thorough extraction of nuclear protein. The sample may be viscous following extraction due to unsheared DNA. To decrease this viscosity, we recommend increasing the volume of GTX400005 and trying either sonication or drawing the sample back-and-forth gently (to avoid bubbles) ten times through an 18G blunt needle attached to a 1ml syringe.