SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263], IgG2a, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263], IgG2a, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX635708
• Hersteller Artikelnummer: GTX635708
• Alternativnummer: GTX635708-100,GTX635708-25
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IHC-P, WB
• Spezies Reaktivität: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) Spike (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: 2019-nCoV Spike, COVID-19 Spike, SARS-CoV-2 Spike, covid-19, sars-cov-2

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [GT263]
• Isotyp: IgG2a
• Sensitivitaet: This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.
• Puffer: PBS, no Preservative.
• Reinheit: Affinity purified by Protein G.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:1000-1:10000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX635708 WB Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) (1 μg/mL). Mouse IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min