SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS], IgG1, unconjugated, mouse, Monoclonal

Artikelnummer: GTX635712
Artikelname: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS], IgG1, unconjugated, mouse, Monoclonal
Artikelnummer: GTX635712
Hersteller Artikelnummer: GTX635712
Alternativnummer: GTX635712-25,GTX635712-100
Hersteller: GeneTex
Wirt: mouse
Kategorie: Antikörper
Applikation: wb, ICC, elisa, IHC-P, ELISA
Spezies Reaktivität: Virus
Immunogen: Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
Konjugation: unconjugated
Alternative Synonym: COVID19, COV-2, COVID-19, 2019-nCoV Nucleocapsid | SARS-CoV-2 Nucleocapsid | COVID-19 Nucleocapsid | 2019-nCoV NP | SARS-CoV-2 NP | COVID-19 NP, COVID, CORONA, SARS-COV, NCOV, COV2, SARS-COV-2
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS], IgG1, Unconjugated, Mouse, Monoclonal
Klonalität: Monoclonal
Konzentration: 0.5mg/ml
Klon-Bezeichnung: HL455-MS
Isotyp: IgG1
Puffer: PBS
Quelle: SARS Coronavirus 2
Reinheit: Affinity purified by Protein A.
Antibody Type: Recombinant Antibody
Anwendungsbeschreibung: WB (1:1000-1:10000), ICC/IF (1:100-1:1000),
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:5000 and used to detect the primary antibody.
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Sensitivity : 2.68 pg/mL
Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 (GTX535665), MERS-CoV and SARS-CoV Nucleocapsid transfected 293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.