ACE2 antibody [GT19410], IgG2b, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: ACE2 antibody [GT19410], IgG2b, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX635897
• Hersteller Artikelnummer: GTX635897
• Alternativnummer: GTX635897-100,GTX635897-25
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IHC-P, LF, WB
• Spezies Reaktivität: Human, Monkey, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACE2. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: angiotensin I converting enzyme 2 , ACEH

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.55 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [GT19410]
• Molekulargewicht: 92
• Isotyp: IgG2b
• NCBI: 59272
• UniProt: Q9BYF1
• Puffer: PBS, no Preservative.
• Reinheit: Affinity purified by Protein A
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX635897 IHC-P Image

SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins. The signal intensity of the test line (T) decreases due to competition by the neutralizing antibody, while the control line (C) demonstrates a valid test.

Neutralizing Antibody: Spike RBD antibody [HL1002] (GTX635791)
C Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: Anti-human ACE2 antibody (GTX635897)
T Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: SARS-CoV-2 Spike RBD protein

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sandwich ELISA detection of recombinant Human ACE2 (ECD) protein, His and Avi tag (active) (GTX01550-pro) using ACE2 antibody [GT19410] (GTX635897) as capture antibody at concentration of 5 μg/mL and 6X His tag antibody [GT359] (HRP) (GTX628914-01) as detection antibody at concentration of 0.1 μg/mL.