TaCLE24b is a root-derived CLAVATA3/Endosperm surrounding region-related (CLE) peptide, which interacts with the leucine-rich repeat receptor-like kinase TaCLV1, which is involved in regulating the development of lateral roots. TaCLE24b enhances the interaction between TaCLV1 and the TaSGD1 gene, leading to increased phosphorylation and degradation of TaSG-D1 and an increase in the density of lateral root and lateral root primordium. The presence of TaCLE24b peptide enhances the binding affinity and phosphorylation ability of TaCLV1 to TaSG-D1, thereby positively fine-tuning lateral root development and drought tolerance.