Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM403B)

Produktübersicht

• Artikelname: Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein (APE-HM403B)
• Artikelnummer: KAB-APE-HM403B
• Hersteller Artikelnummer: APE-HM403B
• Alternativnummer: KAB-APE-HM403B-100UG, KAB-APE-HM403B-500UG
• Hersteller: KactusBio
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Konjugation: Biotin
• Alternative Synonym: Alkaline Phosphatase, ALP-1, Alkaline phosphatase, placental-like, GCAP, PLAP-like, ALPG, ALPPL, ALPPL2

Produktbeschreibung

Recombinant Biotinylated Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Asp503.

Produkteigenschaften

• Molekulargewicht: The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Bis-Tris PAGE result.
• Tag: C-His-Avi
• UniProt: P10696
• Puffer: Lyophilized from 0.22µm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Reinheit: > 95% as determined by Bis-Tris PAGE,> 90% as determined by HPLC
• Formulierung: Lyophilized
• Sequenz: Ile20-Asp503
• Target-Kategorie: Alkaline Phosphatase (Germ type)/ALPG

Bilder

The purity of Biotinylated Human Alkaline Phosphatase (Germ type) is greater than 90% as determined by SEC-HPLC.

Biotinylated Human Alkaline Phosphatase (Germ type) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type), His-Avi Tag with the EC50 of 27.8ng/ml determined by ELISA.

Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Alkaline Phosphatase (Germ Type), His-Avi Tag with an affinity constant of 0.04 nM as determined in BLI assay (Gator Prime).