CD275, B7H2, B7-H2, B7RP-1, GL50, ICOSL, ICOS-L, ICOSLG, B7RP1, LICOS, ICOS ligand, B7-H2B7-related protein 1, B7RP-1, B7RP1B7-like protein Gl50
Recombinant Cynomolgus B7-H2/ICOSLG Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp19-Thr256.
Molekulargewicht:
The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Tag:
C-His
UniProt:
XP_005548617.1
Puffer:
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Asp19-Thr256
Target-Kategorie:
B7-H2/ICOSLG
Human ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).
Immobilized Human ICOS, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-H2, His Tag with the EC50 of 12.6ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus B7-H2 is greater than 95% as determined by SEC-HPLC.
Cynomolgus B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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