Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)

Produktübersicht

• Artikelname: Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)
• Artikelnummer: KAB-CD3-HM557B
• Hersteller Artikelnummer: CD3-HM557B
• Alternativnummer: KAB-CD3-HM557B-100UG, KAB-CD3-HM557B-500UG
• Hersteller: KactusBio
• Kategorie: Proteine/Peptide
• Spezies Reaktivität: Human
• Konjugation: Biotin
• Alternative Synonym: CD3, CD3 epsilon, CD3epsilon, CD3epsilon, CD3 epsilon, CD3E, CD3e, CD3 gamma, CD3gamma, CD3gamma, CD3 gamma, CD3G, CD3g, CD3epsilon&CD3gamma, CD3 epsilon&CD3 gamma, CD3 epsilon & CD3 gamma, CD3epsilon&CD3gamma, CD3 epsilon&CD3 gamma, CD3 epsilon & CD3 gamma, CD3E&CD3G, CD3E & CD3G, CD3 E & CD3 G, CD3e&CD3g, CD3e & CD3g, CD3 e & CD3 g

Produktbeschreibung

Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus of CD3E and hFc (IgG1) tag at the C-Terminus of CD3G.It contains Asp23-Asp126(CD3E) and Gln23-Ser116(CD3G).

Produkteigenschaften

• Molekulargewicht: The protein has a predicted MW of 40 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
• Tag: C-hFc (IgG1)-Avi
• UniProt: P07766
• Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Expression System: HEK293
• Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
• Formulierung: Lyophilized
• Sequenz: Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
• Target-Kategorie: CD3E&CD3G/CD3 epsilon&CD3 gamma

Bilder

Biotinylated CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test).

Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, hFc-Avi Tag with the EC50 of 0.20ug/ml determined by ELISA.

Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3G, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).