Non-biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428)

Artikelnummer: KAB-CD8-HM428
Artikelname: Non-biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428)
Artikelnummer: KAB-CD8-HM428
Hersteller Artikelnummer: CD8-HM428
Alternativnummer: KAB-CD8-HM428-100UG, KAB-CD8-HM428-500UG
Hersteller: KactusBio
Kategorie: Proteine/Peptide
Spezies Reaktivität: Human, Monkey
Alternative Synonym: CD28 antigen (Tp44), CD28 molecule, CD28, MGC138290
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.
Molekulargewicht: The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Bis-Tris PAGE result.
Tag: N-His-Avi
UniProt: P10747
Puffer: Lyophilized from 0.22 µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Reinheit: > 95% as determined by Bis-Tris PAGE
Formulierung: Lyophilized
Sequenz: Asn19-Pro152
Target-Kategorie: CD28
Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15 nM as determined in a SPR assay (Biacore T200).
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200).
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test).