Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Ala46-Pro217
Target-Kategorie:
Fc gamma RIIB/C (CD32b/c)
The purity of Human Fc gamma RIIB/C is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIB/C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIB/C, His Tag with an affinity constant of 3.46 uM as determined in SPR assay (Biacore T200).
Human Fc gamma RIIB/C, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 16.72 uM as determined in SPR assay (Biacore T200).
Loaded Human Fc gamma RIIB, His Tag on Anti-His-Biosensor can bind Rituximab with an affinity constant of 3.09 µM as determined in BLI assay (Gator Prime).
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