Recombinant Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met113-Leu261 trimer design.
Molekulargewicht:
The protein has a predicted MW of 74.9 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Bis-Tris PAGE result.
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Met113-Leu261
Target-Kategorie:
CD40 Ligand/TNFSF5
The purity of Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Serial dilutions of Anti-CD40 Antibody were added into Biotinylated Human CD40, His Tag : Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) , hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 2.0ug/ml.
Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque CD40 Ligand (Trimer), hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test).
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